The risk created by infectious microorganisms to humans attracted the development\nof common medicine. To find an alternative source, medicinal\nplants with diverse metabolites play an important role in curing the diseases\nand human disorders caused by microbial pathogens. Medicinal plants namely,\nCitrullus colocynthis , Hyoscyamus muticus , Ocimum basilicum , Amaranthus\nlividus , Salvia aegyptiaca and Ruta chalepensis are commonly used as a\ntraditional medicine in Gulf countries. The present study aimed to investigate\nthe antibacterial, antifungal and antioxidant potential of the organic crude extracts\nobtained from the seeds. Besides, the possible antimicrobial mechanisms\nof the extracts were evaluated by determining the enzyme activities. The\nantibacterial and antifungal activities of the crude extracts were evaluated by\nthe broth micro dilution method and the effect of the extracts on the pathogens\nwere determined by quantifying the alkaline phosphatase (ALP), lactate\ndehydrogenase (LDH) enzymes and intracellular protein leakage. Besides, the\nantioxidant properties were determined using hydroxyl radical scavenging assay,\nDPPH radical scavenging assay, reducing power assay and superoxide\nradical scavenging assay. Results indicated that the extracts of C. colocynthis\nshowed promising activity against all the tested pathogens, especially the MIC\nvalues were ranged from 100 to 150 �¼g/ml for Gram positive bacteria and 100\nto 250 �¼g/ml for Gram negative bacteria respectively. The MIC values of H.\nmuticus , O. basilicum and R. chalepensis against the fungal pathogens were\nranged from 100 to 500 �¼g/mL respectively. The ALP activity was higher in\nextract treated Klebsiella pneumoniae compared with control, whereas the\nLDH and protein concentrations for Escherichia coli and Staphylococcus aureus\nwere comparatively higher. Furthermore, all the studied seed extract\nshowed good antioxidant activities. In conclusion, the studied plant seed extracts\ndocumented good antimicrobial and antioxidant activities. Therefore,\nthe medicinal plants would be the excellent source for natural antioxidant and antibacterial agents for medical and applications.
Loading....